all you need to know about
CLONING
ANIMAL CLONING
There are basically three type of cloning we are going to discuss: DNA cloning,
reproductive cloning and therapeutic cloning.
DNA
Cloning
DNA
cloning is also known as recombinant DNA technology or gene cloning. Only the DNA of the
cell is extracted and replicated. In this cloning technique, we use a self-replicating
genetic element called plasmid as the main tools. Plasmid can be extracted from any
bacterial cells and DNA is transferred and replicates using this element.
In
other words, the interested DNA fragment is cut and joins back with a plasmid. Plasmid can
reproduces itself to many copies with same DNA information. Plasmid we use in this
technique is known as a vector. The multiple copies of DNA can be individually introduce
into the host cell and keep for need.
Reproductive
Cloning
Reproductive
cloning is a technology used to generate an animal that has the same nuclear DNA as
another currently or previously existing animal. The famous clone, Dolly was created by
reproductive cloning technology. The DNA information from the nucleus of the donor adult
cell is transfers into a cell whose nucleus that has been remove. This process is called
somatic cell nucleus transfer. Chemicals or electric current is used to
stimulate the cell division. Once the cell starts dividing and grows into embryo stage, it
is implanted into the uterus of the host where there it develops until birth. Generally it
is the practice of creating or attempting to create a human being by transferring the
nucleus from one donor cell into an egg cell from which the nucleus has been removed,
implanting the resulting product in a uterus to initiate a pregnancy.
Therapeutic
Cloning
Therapeutic cloning is like reproductive cloning, except the embryonic cells are not
allowed to develop fully. It involves somatic
cell nuclear transfer for the isolation of embryonic stem cells in vitro. It also known as
stem cell therapy. The basic idea is to take the healthy cells from a person and create
many copies that serve as the source of replace or repair damaged tissues and organs. The main purpose is to harvest stem
cells that can be used for study human development and treat disease or we call that
medical treatment. Stem cells are important in this technique because they can be used to
generate any type of specialized cell in the human body. The main goal is to produce
genetically-matched tissues for therapeutic use.
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